High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA

We describe a rapid and reproducible method for assessment of the hepatitis C virus (HCV) load in serum samples. The method combines Taqman technology (Roche) and the ABI Prism 7700 (Perkin Elmer) real-time sequence detection system. We have optimized a single-tube reverse transcription-PCR (RT-PCR)...

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Detalles Bibliográficos
Main Authors: Martell, María, Gómez, Jordi, Esteban, Juan I., Sauleda, Silvia, Quer, Josep, Cabot, Beatriz, Esteban, Rafael, Guardia, Jaime
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 1999
Assuntos:
Virology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC84298/
https://ncbi.nlm.nih.gov/pubmed/9889212
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