High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA

We describe a rapid and reproducible method for assessment of the hepatitis C virus (HCV) load in serum samples. The method combines Taqman technology (Roche) and the ABI Prism 7700 (Perkin Elmer) real-time sequence detection system. We have optimized a single-tube reverse transcription-PCR (RT-PCR)...

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Dades bibliogràfiques

Publicat a:	J Clin Microbiol
Autors principals:	Martell, María, Gómez, Jordi, Esteban, Juan I., Sauleda, Silvia, Quer, Josep, Cabot, Beatriz, Esteban, Rafael, Guardia, Jaime
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 1999
Matèries:	Virology
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC84298/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9889212/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.37.2.327-332.1999
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