High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA
We describe a rapid and reproducible method for assessment of the hepatitis C virus (HCV) load in serum samples. The method combines Taqman technology (Roche) and the ABI Prism 7700 (Perkin Elmer) real-time sequence detection system. We have optimized a single-tube reverse transcription-PCR (RT-PCR)...
Uloženo v:
| Vydáno v: | J Clin Microbiol |
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| Hlavní autoři: | , , , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology (ASM)
1999
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC84298/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9889212/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.37.2.327-332.1999 |
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