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High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA

We describe a rapid and reproducible method for assessment of the hepatitis C virus (HCV) load in serum samples. The method combines Taqman technology (Roche) and the ABI Prism 7700 (Perkin Elmer) real-time sequence detection system. We have optimized a single-tube reverse transcription-PCR (RT-PCR)...

詳細記述

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書誌詳細
出版年:J Clin Microbiol
主要な著者: Martell, María, Gómez, Jordi, Esteban, Juan I., Sauleda, Silvia, Quer, Josep, Cabot, Beatriz, Esteban, Rafael, Guardia, Jaime
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology (ASM) 1999
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC84298/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9889212/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.37.2.327-332.1999
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