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Protocol to apply spike-in ChIP-seq to capture massive histone acetylation in human cells
Inhibition of histone deacetylases causes rapid and robust acetylation of histones. In this case, histone acetylation is likely increased on nearly every nucleosome, and the per-cell DNA/chromatin yield in chromatin immunoprecipitation (ChIP) experiments is significantly increased. Spike-in controls...
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| Vydáno v: | STAR Protoc |
|---|---|
| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Elsevier
2021
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8313745/ https://ncbi.nlm.nih.gov/pubmed/34337446 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100681 |
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