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Protocol to apply spike-in ChIP-seq to capture massive histone acetylation in human cells

Inhibition of histone deacetylases causes rapid and robust acetylation of histones. In this case, histone acetylation is likely increased on nearly every nucleosome, and the per-cell DNA/chromatin yield in chromatin immunoprecipitation (ChIP) experiments is significantly increased. Spike-in controls...

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Bibliografische gegevens
Gepubliceerd in:STAR Protoc
Hoofdauteurs: Wu, Di, Wang, Liguo, Huang, Haojie
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier 2021
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8313745/
https://ncbi.nlm.nih.gov/pubmed/34337446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100681
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