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Protocol to apply spike-in ChIP-seq to capture massive histone acetylation in human cells
Inhibition of histone deacetylases causes rapid and robust acetylation of histones. In this case, histone acetylation is likely increased on nearly every nucleosome, and the per-cell DNA/chromatin yield in chromatin immunoprecipitation (ChIP) experiments is significantly increased. Spike-in controls...
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| Опубликовано в: : | STAR Protoc |
|---|---|
| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Elsevier
2021
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8313745/ https://ncbi.nlm.nih.gov/pubmed/34337446 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100681 |
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