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Protocol to apply spike-in ChIP-seq to capture massive histone acetylation in human cells

Inhibition of histone deacetylases causes rapid and robust acetylation of histones. In this case, histone acetylation is likely increased on nearly every nucleosome, and the per-cell DNA/chromatin yield in chromatin immunoprecipitation (ChIP) experiments is significantly increased. Spike-in controls...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Wu, Di, Wang, Liguo, Huang, Haojie
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8313745/
https://ncbi.nlm.nih.gov/pubmed/34337446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100681
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