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Analysis of electrostatic coupling throughout the laboratory evolution of a designed retroaldolase
The roles of local interactions in the laboratory evolution of a highly active, computationally designed retroaldolase (RA) are examined. Partial Order Optimum Likelihood (POOL) is used to identify catalytically important amino acid interactions in several RA95 enzyme variants. The series RA95.5, RA...
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| Publicado en: | Protein Sci |
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| Autores principales: | , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
John Wiley & Sons, Inc.
2021
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8284568/ https://ncbi.nlm.nih.gov/pubmed/33938058 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.4099 |
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