Single-molecule FRET imaging of GPCR dimers in living cells

Class C G protein-coupled receptors (GPCRs) are known to form stable homodimers or heterodimers critical for function, but the oligomeric status of class A and B receptors, which constitute >90% of all GPCRs, remains hotly debated. Single-molecule fluorescence resonance energy transfer (smFRET) i...

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Udgivet i:Nat Methods
Main Authors: Asher, Wesley B., Geggier, Peter, Holsey, Michael D., Gilmore, Grant T., Pati, Avik K., Meszaros, Jozsef, Terry, Daniel S., Mathiasen, Signe, Kaliszewski, Megan J., McCauley, Mitchell D., Govindaraju, Alekhya, Zhou, Zhou, Harikumar, Kaleeckal G., Jaqaman, Khuloud, Miller, Laurence J., Smith, Adam W., Blanchard, Scott C., Javitch, Jonathan A.
Format: Artigo
Sprog:Inglês
Udgivet: 2021
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8232828/
https://ncbi.nlm.nih.gov/pubmed/33686301
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-021-01081-y
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