Protocol for using heterologous spike-ins to normalize for technical variation in chromatin immunoprecipitation

Quantifying differential genome occupancy by chromatin immunoprecipitation (ChIP) remains challenging due to variation in chromatin fragmentation, immunoprecipitation efficiencies, and intertube variability. In this protocol, we add heterologous spike-ins from Drosophila chromatin as an internal con...

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Foilsithe in:STAR Protoc
Main Authors: Greulich, Franziska, Mechtidou, Aikaterini, Horn, Teresa, Uhlenhaut, Nina Henriette
Formáid: Artigo
Teanga:Inglês
Foilsithe: Elsevier 2021
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Protocol
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8220248/
https://ncbi.nlm.nih.gov/pubmed/34189474
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100609
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