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Protocol for using heterologous spike-ins to normalize for technical variation in chromatin immunoprecipitation
Quantifying differential genome occupancy by chromatin immunoprecipitation (ChIP) remains challenging due to variation in chromatin fragmentation, immunoprecipitation efficiencies, and intertube variability. In this protocol, we add heterologous spike-ins from Drosophila chromatin as an internal con...
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| Gepubliceerd in: | STAR Protoc |
|---|---|
| Hoofdauteurs: | , , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Elsevier
2021
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8220248/ https://ncbi.nlm.nih.gov/pubmed/34189474 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100609 |
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