Optimization of reaction condition of recombinase polymerase amplification to detect SARS-CoV-2 DNA and RNA using a statistical method

Recombinase polymerase amplification (RPA) is an isothermal reaction that amplifies a target DNA sequence with a recombinase, a single-stranded DNA-binding protein (SSB), and a strand-displacing DNA polymerase. In this study, we optimized the reaction conditions of RPA to detect SARS-CoV-2 DNA and R...

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Udgivet i:Biochem Biophys Res Commun
Main Authors: Juma, Kevin Maafu, Takita, Teisuke, Ito, Kenji, Yamagata, Masaya, Akagi, Shihomi, Arikawa, Emi, Kojima, Kenji, Biyani, Manish, Fujiwara, Shinsuke, Nakura, Yukiko, Yanagihara, Itaru, Yasukawa, Kiyoshi
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier Inc. 2021
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8189764/
https://ncbi.nlm.nih.gov/pubmed/34166918
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2021.06.023
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