Reversible RNA acylation for control of CRISPR–Cas9 gene editing

We report the development of post-transcriptional chemical methods that enable control over CRISPR–Cas9 gene editing activity both in in vitro assays and in living cells. We show that an azide-substituted acyl imidazole reagent (NAI-N(3)) efficiently acylates CRISPR single guide RNAs (sgRNAs) in 20...

Descripció completa

Guardat en:
Dades bibliogràfiques
Publicat a:Chem Sci
Autors principals: Habibian, Maryam, McKinlay, Colin, Blake, Timothy R., Kietrys, Anna M., Waymouth, Robert M., Wender, Paul A., Kool, Eric T.
Format: Artigo
Idioma:Inglês
Publicat: The Royal Society of Chemistry 2019
Matèries:
Chemistry
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8145180/
https://ncbi.nlm.nih.gov/pubmed/34084356
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c9sc03639c
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!

Ítems similars