A Survey of Validation Strategies for CRISPR-Cas9 Editing

The T7 endonuclease 1 (T7E1) mismatch detection assay is a widely used method for evaluating the activity of site-specific nucleases, such as the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system. To determine the accuracy and sensitivity of this assay, we compared the e...

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Udgivet i:Sci Rep
Main Authors: Sentmanat, Monica F., Peters, Samuel T., Florian, Colin P., Connelly, Jon P., Pruett-Miller, Shondra M.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2018
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5772360/
https://ncbi.nlm.nih.gov/pubmed/29343825
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-19441-8
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