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In Vitro Pre-validation of Gene Editing by CRISPR/Cas9 Ribonucleoprotein

BACKGROUND: The CRISPR/Cas9 genome editing system is a powerful and simple gene editing method. The format of the CRISPR components is one of the important factors in targeting efficiency. Compared to plasmid or mRNA (IVTs) format, using the CRISPR/Cas9 system as Cas9–crRNA–tracrRNA RNP format is mo...

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Bibliographic Details
Published in:Avicenna J Med Biotechnol
Main Authors: Mehravar, Maryam, Shirazi, Abolfazl, Mehrazar, Mohammad Mehdi, Nazari, Mahboobeh
Format: Artigo
Language:Inglês
Published: Avicenna Research Institute 2019
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6626505/
https://ncbi.nlm.nih.gov/pubmed/31380000
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