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Efficient Production of Biallelic RAG1 Knockout Mouse Embryonic Stem Cell Using CRISPR/Cas9

BACKGROUND: Recombination Activating Genes (RAG) mutated embryonic stem cells are (ES) cells which are unable to perform V (D) J recombination. These cells can be used for generation of immunodeficient mouse. Creating biallelic mutations by CRISPR/Cas9 genome editing has emerged as a powerful techni...

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Detalhes bibliográficos
Publicado no:Iran J Biotechnol
Main Authors: Mehravar, Maryam, Shirazi, Abolfazl, Mehrazar, Mohammad Mehdi, Nazari, Mahboobeh, Banan, Mehdi, Salimi, Maryam
Formato: Artigo
Idioma:Inglês
Publicado em: National Institute of Genetic Engineering and Biotechnology 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6697863/
https://ncbi.nlm.nih.gov/pubmed/31457047
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21859/ijb.2205
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