A Fast and Efficient Single-stranded Genomic Library Preparation Method Optimized for Ancient DNA

We present a protocol to prepare extracted DNA for sequencing on the Illumina sequencing platform that has been optimized for ancient and degraded DNA. Our approach, the Santa Cruz Reaction or SCR, uses directional splinted ligation of Illumina’s P5 and P7 adapters to convert natively single-strande...

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Bibliographic Details
Published in:J Hered
Main Authors: Kapp, Joshua D, Green, Richard E, Shapiro, Beth
Format: Artigo
Language:Inglês
Published: Oxford University Press 2021
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Original Articles
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC8141684/
https://ncbi.nlm.nih.gov/pubmed/33768239
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/jhered/esab012
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