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A Fast and Efficient Single-stranded Genomic Library Preparation Method Optimized for Ancient DNA
We present a protocol to prepare extracted DNA for sequencing on the Illumina sequencing platform that has been optimized for ancient and degraded DNA. Our approach, the Santa Cruz Reaction or SCR, uses directional splinted ligation of Illumina’s P5 and P7 adapters to convert natively single-strande...
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| Publicado en: | J Hered |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2021
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8141684/ https://ncbi.nlm.nih.gov/pubmed/33768239 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/jhered/esab012 |
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