Single-stranded DNA library preparation from highly degraded DNA using T4 DNA ligase

DNA library preparation for high-throughput sequencing of genomic DNA usually involves ligation of adapters to double-stranded DNA fragments. However, for highly degraded DNA, especially ancient DNA, library preparation has been found to be more efficient if each of the two DNA strands are converted...

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Podrobná bibliografie
Vydáno v:Nucleic Acids Res
Hlavní autoři: Gansauge, Marie-Theres, Gerber, Tobias, Glocke, Isabelle, Korlević, Petra, Lippik, Laurin, Nagel, Sarah, Riehl, Lara Maria, Schmidt, Anna, Meyer, Matthias
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2017
Témata:
Methods Online
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5449542/
https://ncbi.nlm.nih.gov/pubmed/28119419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx033
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