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Ligase-mediated construction of branched DNA strands: a novel DNA joining activity catalyzed by T4 DNA ligase

Branched nucleic acid strands exist as intermediates in certain biological reactions, and bifurcating DNA also presents interesting opportunities in biotechnological applications. We describe here how T4 DNA ligase can be used for efficient construction of DNA molecules having one 5′ end but two dis...

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Bibliografiska uppgifter
Huvudupphovsmän: Mendel-Hartvig, Maritha, Kumar, Anil, Landegren, Ulf
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2004
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC373312/
https://ncbi.nlm.nih.gov/pubmed/14704359
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh011
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