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Ligase-mediated construction of branched DNA strands: a novel DNA joining activity catalyzed by T4 DNA ligase

Branched nucleic acid strands exist as intermediates in certain biological reactions, and bifurcating DNA also presents interesting opportunities in biotechnological applications. We describe here how T4 DNA ligase can be used for efficient construction of DNA molecules having one 5′ end but two dis...

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書誌詳細
主要な著者: Mendel-Hartvig, Maritha, Kumar, Anil, Landegren, Ulf
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 2004
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC373312/
https://ncbi.nlm.nih.gov/pubmed/14704359
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh011
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