Ligase-mediated construction of branched DNA strands: a novel DNA joining activity catalyzed by T4 DNA ligase

Branched nucleic acid strands exist as intermediates in certain biological reactions, and bifurcating DNA also presents interesting opportunities in biotechnological applications. We describe here how T4 DNA ligase can be used for efficient construction of DNA molecules having one 5′ end but two dis...

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Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Mendel-Hartvig, Maritha, Kumar, Anil, Landegren, Ulf
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 2004
Témata:	NAR Methods Online
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC373312/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14704359/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh011
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