Specific and reversible inhibition of the blunt end joining activity of the T4 DNA ligase.
Specific, complete and reversible inhibition of the joining of blunt ended DNA duplexes catalyzed by the T4 DNA ligase can be obtained by using ATP, the enzyme cofactor, at concentrations of 5 mM and higher. On cohesive DNA ends, 5 mM ATP, which completely inhibits blunt end ligation, brings about o...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1981
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327385/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6269089/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.15.3695 |
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