Specific and reversible inhibition of the blunt end joining activity of the T4 DNA ligase.

Specific, complete and reversible inhibition of the joining of blunt ended DNA duplexes catalyzed by the T4 DNA ligase can be obtained by using ATP, the enzyme cofactor, at concentrations of 5 mM and higher. On cohesive DNA ends, 5 mM ATP, which completely inhibits blunt end ligation, brings about o...

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Detaylı Bibliyografya
Asıl Yazarlar: Ferretti, L, Sgaramella, V
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1981
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC327385/
https://ncbi.nlm.nih.gov/pubmed/6269089
Etiketler: Etiketle
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