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High-throughput functional variant screens via in vivo production of single-stranded DNA
Creating and characterizing individual genetic variants remains limited in scale, compared to the tremendous variation both existing in nature and envisioned by genome engineers. Here we introduce retron library recombineering (RLR), a methodology for high-throughput functional screens that surpasse...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
2021
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8106316/ https://ncbi.nlm.nih.gov/pubmed/33906944 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2018181118 |
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