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High-throughput functional variant screens via in vivo production of single-stranded DNA
Creating and characterizing individual genetic variants remains limited in scale, compared to the tremendous variation both existing in nature and envisioned by genome engineers. Here we introduce retron library recombineering (RLR), a methodology for high-throughput functional screens that surpasse...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
2021
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8106316/ https://ncbi.nlm.nih.gov/pubmed/33906944 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2018181118 |
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