Sparse isotope labeling for nuclear magnetic resonance (NMR) of glycoproteins using (13)C-glucose

Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as (13)C and (15)N. This is routine for proteins that can be expressed in bacterial culture where low-cost isotopically enriched metabolic...

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Foilsithe in:Glycobiology
Main Authors: Rogals, Monique J, Yang, Jeong-Yeh, Williams, Robert V, Moremen, Kelley W, Amster, I Jonathan, Prestegard, James H
Formáid: Artigo
Teanga:Inglês
Foilsithe: Oxford University Press 2020
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Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8091466/
https://ncbi.nlm.nih.gov/pubmed/32902634
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/glycob/cwaa071
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