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NMR Resonance Assignments of Sparsely Labeled Proteins: Amide Proton Exchange Correlations in Native and Denatured States

Protein NMR assignments of large proteins using traditional triple resonance techniques depends on double or triple labeling of samples with (15)N, (13)C, and (2)H. This is not always practical with proteins that require expression in non-bacterial hosts. Labeling with isotopically labeled versions...

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Detalhes bibliográficos
Main Authors: Nkari, Wendy K., Prestegard, James H.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2699400/
https://ncbi.nlm.nih.gov/pubmed/19317468
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja8100775
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