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NMR Resonance Assignments of Sparsely Labeled Proteins: Amide Proton Exchange Correlations in Native and Denatured States

Protein NMR assignments of large proteins using traditional triple resonance techniques depends on double or triple labeling of samples with (15)N, (13)C, and (2)H. This is not always practical with proteins that require expression in non-bacterial hosts. Labeling with isotopically labeled versions...

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Autors principals: Nkari, Wendy K., Prestegard, James H.
Format: Artigo
Idioma:Inglês
Publicat: 2009
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2699400/
https://ncbi.nlm.nih.gov/pubmed/19317468
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja8100775
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