A flow-cytometry-based protocol for detection of mitochondrial ROS production under hypoxia

Hypoxia is known to stimulate mitochondrial reactive oxygen species (mROS) in cells. Here, we present a detailed protocol to detect mROS using MitoSOX staining in live cells under normoxia and hypoxia. Flow cytometry allows sensitive and reliable quantification of mROS by FlowJo software. We optimiz...

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Publicat a:STAR Protoc
Autors principals: Yang, Yun, Zhang, Guimin, Yang, Tao, Gan, Jia, Xu, Lin, Yang, Hanshuo
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2021
Matèries:
Protocol
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8086139/
https://ncbi.nlm.nih.gov/pubmed/33997804
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100466
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