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A flow-cytometry-based protocol for detection of mitochondrial ROS production under hypoxia
Hypoxia is known to stimulate mitochondrial reactive oxygen species (mROS) in cells. Here, we present a detailed protocol to detect mROS using MitoSOX staining in live cells under normoxia and hypoxia. Flow cytometry allows sensitive and reliable quantification of mROS by FlowJo software. We optimiz...
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| Udgivet i: | STAR Protoc |
|---|---|
| Main Authors: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Elsevier
2021
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8086139/ https://ncbi.nlm.nih.gov/pubmed/33997804 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100466 |
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