A flow-cytometry-based protocol for detection of mitochondrial ROS production under hypoxia

Hypoxia is known to stimulate mitochondrial reactive oxygen species (mROS) in cells. Here, we present a detailed protocol to detect mROS using MitoSOX staining in live cells under normoxia and hypoxia. Flow cytometry allows sensitive and reliable quantification of mROS by FlowJo software. We optimiz...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:STAR Protoc
Päätekijät: Yang, Yun, Zhang, Guimin, Yang, Tao, Gan, Jia, Xu, Lin, Yang, Hanshuo
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Elsevier 2021
Aiheet:
Protocol
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC8086139/
https://ncbi.nlm.nih.gov/pubmed/33997804
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100466
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