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MitoSOX-Based Flow Cytometry for Detecting Mitochondrial ROS
MitoSOX-based assays are widely used to detect mitochondrial reactive oxygen species (ROS), especially superoxide. To this end, 5 μM MitoSOX is commonly used. In this ROS Protocols article, we described the flow cytometric protocol involving the use of various concentrations of MitoSOX (1, 2.5, 5 μM...
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| Udgivet i: | React Oxyg Species (Apex) |
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| Main Authors: | , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2016
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5926237/ https://ncbi.nlm.nih.gov/pubmed/29721549 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.20455/ros.2016.865 |
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