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A flow-cytometry-based protocol for detection of mitochondrial ROS production under hypoxia

Hypoxia is known to stimulate mitochondrial reactive oxygen species (mROS) in cells. Here, we present a detailed protocol to detect mROS using MitoSOX staining in live cells under normoxia and hypoxia. Flow cytometry allows sensitive and reliable quantification of mROS by FlowJo software. We optimiz...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:STAR Protoc
Hauptverfasser: Yang, Yun, Zhang, Guimin, Yang, Tao, Gan, Jia, Xu, Lin, Yang, Hanshuo
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2021
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8086139/
https://ncbi.nlm.nih.gov/pubmed/33997804
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100466
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