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Footprinting SHAPE-eCLIP reveals transcriptome-wide hydrogen bonds at RNA-protein interfaces

Discovering the interaction mechanism and location of RNA binding proteins (RBPs) on RNA is critical for understanding gene expression regulation. Here, we apply selective 2’-hydroxyl acylation analyzed by primer extension (SHAPE) on in vivo transcripts compared to protein-absent transcripts in four...

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Publicat a:Mol Cell
Autors principals: Corley, Meredith, Flynn, Ryan A, Lee, Byron, Blue, Steven M, Chang, Howard Y, Yeo, Gene W
Format: Artigo
Idioma:Inglês
Publicat: 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8074864/
https://ncbi.nlm.nih.gov/pubmed/33242392
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2020.11.014
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