Immuno-profiling and cellular spatial analysis using five immune oncology multiplex immunofluorescence panels for paraffin tumor tissue

Multiplex immunofluorescence (mIF) has arisen as an important tool for immuno-profiling tumor tissues. We updated our manual protocol into an automated protocol that allows the use of up to seven markers in five mIF panels to apply to formalin-fixed paraffin-embedded tumor tissues. Using a tyramide...

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Veröffentlicht in:Sci Rep
Hauptverfasser: Parra, Edwin Roger, Ferrufino-Schmidt, Maria C., Tamegnon, Auriole, Zhang, Jiexin, Solis, Luisa, Jiang, Mei, Ibarguen, Heladio, Haymaker, Cara, Lee, J. Jack, Bernatchez, Chantale, Wistuba, Ignacio Ivan
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2021
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8055659/
https://ncbi.nlm.nih.gov/pubmed/33875760
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-88156-0
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