Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues

Immune-profiling is becoming an important tool to identify predictive markers for the response to immunotherapy. Our goal was to validate multiplex immunofluorescence (mIF) panels to apply to formalin-fixed and paraffin-embedded tissues using a set of immune marker antibodies, with the Opal™ 7 color...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Parra, Edwin R., Uraoka, Naohiro, Jiang, Mei, Cook, Pamela, Gibbons, Don, Forget, Marie-Andrée, Bernatchez, Chantale, Haymaker, Cara, Wistuba, Ignacio I., Rodriguez-Canales, Jaime
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5645415/
https://ncbi.nlm.nih.gov/pubmed/29042640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-13942-8
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