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The V(H) framework region 1 as a target of efficient mutagenesis for generating a variety of affinity-matured scFv mutants
In vitro affinity-maturation potentially generates antibody fragments with enhanced antigen-binding affinities that allow for developing more sensitive diagnostic systems and more effective therapeutic agents. Site-directed mutagenesis targeting “hot regions,” i.e., amino acid substitutions therein...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8050046/ https://ncbi.nlm.nih.gov/pubmed/33859250 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-87501-7 |
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