CYP154C5 Regioselectivity in Steroid Hydroxylation Explored by Substrate Modifications and Protein Engineering

CYP154C5 from Nocardia farcinica is a P450 monooxygenase able to hydroxylate a range of steroids with high regio‐ and stereoselectivity at the 16α‐position. Using protein engineering and substrate modifications based on the crystal structure of CYP154C5, an altered regioselectivity of the enzyme in...

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Dettagli Bibliografici
Pubblicato in:Chembiochem
Autori principali: Bracco, Paula, Wijma, Hein J., Nicolai, Bastian, Buitrago, Jhon Alexander Rodriguez, Klünemann, Thomas, Vila, Agustina, Schrepfer, Patrick, Blankenfeldt, Wulf, Janssen, Dick B., Schallmey, Anett
Natura: Artigo
Lingua:Inglês
Pubblicazione: John Wiley and Sons Inc. 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC8048783/
https://ncbi.nlm.nih.gov/pubmed/33145893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.202000735
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