A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs

Detailed target-selectivity information and experiment-based efficacy prediction tools are primarily available for Streptococcus pyogenes Cas9 (SpCas9). One obstacle to develop such tools is the rarity of accurate data. Here, we report a method termed ‘Self-targeting sgRNA Library Screen’ (SLS) for...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Tálas, András, Huszár, Krisztina, Kulcsár, Péter István, Varga, Julia K, Varga, Éva, Tóth, Eszter, Welker, Zsombor, Erdős, Gergely, Pach, Péter Ferenc, Welker, Ágnes, Györgypál, Zoltán, Tusnády, Gábor E, Welker, Ervin
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2021
Fag:
Methods Online
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8034649/
https://ncbi.nlm.nih.gov/pubmed/33450024
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa1220
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