Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases

The widespread use of Cas12a (formerly Cpf1) nucleases for genome engineering is limited by their requirement for a rather long TTTV protospacer adjacent motif (PAM) sequence. Here we have aimed to loosen these PAM constraints and have generated new PAM mutant variants of the four Cas12a orthologs t...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Tóth, Eszter, Varga, Éva, Kulcsár, Péter István, Kocsis-Jutka, Virág, Krausz, Sarah Laura, Nyeste, Antal, Welker, Zsombor, Huszár, Krisztina, Ligeti, Zoltán, Tálas, András, Welker, Ervin
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7144938/
https://ncbi.nlm.nih.gov/pubmed/32107556
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa110
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