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DNA Double-Strand Breaks Affect Chromosomal Rearrangements during Methotrexate-Mediated Gene Amplification in Chinese Hamster Ovary Cells

Methotrexate (MTX)-mediated gene amplification has been widely used in Chinese hamster ovary (CHO) cells for the biomanufacturing of therapeutic proteins. Although many studies have reported chromosomal instability and extensive chromosomal rearrangements in MTX-mediated gene-amplified cells, which...

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Publicado en:Pharmaceutics
Autores principales: Baik, Jong Youn, Han, Hye-Jin, Lee, Kelvin H.
Formato: Artigo
Lenguaje:Inglês
Publicado: MDPI 2021
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC8000239/
https://ncbi.nlm.nih.gov/pubmed/33809068
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/pharmaceutics13030376
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