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Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR–Cas12a gRNA switch
CRISPR technologies increasingly require spatiotemporal and dosage control of nuclease activity. One promising strategy involves linking nuclease activity to a cell's transcriptional state by engineering guide RNAs (gRNAs) to function only after complexing with a ‘trigger’ RNA. However, standar...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7968991/ https://ncbi.nlm.nih.gov/pubmed/33619539 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkab100 |
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