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The Francisella novicida Cas12a is sensitive to the structure downstream of the terminal repeat in CRISPR arrays

The Class 2 Type V-A CRISPR effector protein Cas12a/Cpf1 has gained widespread attention in part because of the ease in achieving multiplexed genome editing, gene regulation, and DNA detection. Multiplexing derives from the ability of Cas12a alone to generate multiple guide RNAs from a transcribed C...

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Detalhes bibliográficos
Publicado no:RNA Biol
Main Authors: Liao, Chunyu, Slotkowski, Rebecca A., Achmedov, Tatjana, Beisel, Chase L.
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6546362/
https://ncbi.nlm.nih.gov/pubmed/30252595
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2018.1526537
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