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Toward a translationally independent RNA-based synthetic oscillator using deactivated CRISPR-Cas
In synthetic circuits, CRISPR-Cas systems have been used effectively for endpoint changes from an initial state to a final state, such as in logic gates. Here, we use deactivated Cas9 (dCas9) and deactivated Cas12a (dCas12a) to construct dynamic RNA ring oscillators that cycle continuously between s...
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| Publicado en: | Nucleic Acids Res |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2020
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7430638/ https://ncbi.nlm.nih.gov/pubmed/32609820 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa557 |
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