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Synthetic switch to minimize CRISPR off-target effects by self-restricting Cas9 transcription and translation
CRISPR/Cas9 is a powerful genome editing system but uncontrolled Cas9 nuclease expression triggers off-target effects and even in vivo immune responses. Inspired by synthetic biology, here we built a synthetic switch that self-regulates Cas9 expression not only in the transcription step by guide RNA...
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| Опубликовано в: : | Nucleic Acids Res |
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| Главные авторы: | , , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
2019
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6379646/ https://ncbi.nlm.nih.gov/pubmed/30462300 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky1165 |
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