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One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis

Xenoantigens cause hyperacute rejection and limit the success of interspecific xenografts. Therefore, genes involved in xenoantigen biosynthesis, such as GGTA1, CMAH, and B4GALNT2, are key targets to improve the outcomes of xenotransplantation. In this study, we introduced a CRISPR/Cas9 system simul...

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Detalhes bibliográficos
Publicado no:Int J Mol Sci
Main Authors: Tanihara, Fuminori, Hirata, Maki, Nguyen, Nhien Thi, Sawamoto, Osamu, Kikuchi, Takeshi, Otoi, Takeshige
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7956194/
https://ncbi.nlm.nih.gov/pubmed/33668187
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms22052249
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