Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages

OBJECTIVE: Cytoplasmic microinjection and electroporation of the CRISPR/Cas9 system into zygotes are used for generating genetically modified pigs. However, these methods create mosaic mutations in embryos. In this study, we evaluated whether the gene editing method and embryonic stage for gene edit...

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Detalhes bibliográficos
Publicado no:BMC Res Notes
Main Authors: Le, Quynh Anh, Tanihara, Fuminori, Wittayarat, Manita, Namula, Zhao, Sato, Yoko, Lin, Qingyi, Takebayashi, Koki, Hirata, Maki, Otoi, Takeshige
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2021
Assuntos:
Research Note
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7788904/
https://ncbi.nlm.nih.gov/pubmed/33407863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-020-05412-8
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