Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages

OBJECTIVE: Cytoplasmic microinjection and electroporation of the CRISPR/Cas9 system into zygotes are used for generating genetically modified pigs. However, these methods create mosaic mutations in embryos. In this study, we evaluated whether the gene editing method and embryonic stage for gene edit...

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Bibliografiske detaljer
Udgivet i:BMC Res Notes
Main Authors: Le, Quynh Anh, Tanihara, Fuminori, Wittayarat, Manita, Namula, Zhao, Sato, Yoko, Lin, Qingyi, Takebayashi, Koki, Hirata, Maki, Otoi, Takeshige
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2021
Fag:
Research Note
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7788904/
https://ncbi.nlm.nih.gov/pubmed/33407863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-020-05412-8
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