Genome editing of porcine zygotes via lipofection of two guide RNAs using a CRISPR/Cas9 system

CRISPR/Cas9-based multiplex genome editing via electroporation is relatively efficient; however, lipofection is versatile because of its ease of use and low cost. Here, we aimed to determine the efficiency of lipofection in CRISPR/Cas9-based multiplex genome editing using growth hormone receptor (GH...

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Autori principali:	Qingyi LIN, Koki TAKEBAYASHI, Nanaka TORIGOE, Bin LIU, Zhao NAMULA, Maki HIRATA, Fuminori TANIHARA, Megumi NAGAHARA, Takeshige OTOI
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	The Society for Reproduction and Development 2024-08-01
Serie:	The Journal of Reproduction and Development
Soggetti:	crispr/cas9 system growth hormone receptor (ghr) glycoprotein alpha-galactosyltransferase 1 (ggta1) lipofection porcine zygote
Accesso online:	https://www.jstage.jst.go.jp/article/jrd/70/6/70_2024-054/_pdf/-char/en
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