Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation

TP53 (which encodes p53) is one of the most frequently mutated genes in cancers. In this study, we generated TP53-mutant pigs by gene editing via electroporation of the Cas9 protein (GEEP), a process that involves introducing the Cas9 protein and single-guide RNA (sgRNA) targeting exon 3 and intron...

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Enregistré dans:
Détails bibliographiques
Publié dans:PLoS One
Auteurs principaux: Tanihara, Fuminori, Hirata, Maki, Nguyen, Nhien Thi, Le, Quynh Anh, Hirano, Takayuki, Takemoto, Tatsuya, Nakai, Michiko, Fuchimoto, Dai-ichiro, Otoi, Takeshige
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2018
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6198999/
https://ncbi.nlm.nih.gov/pubmed/30352075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0206360
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