Engineered miniature H1 promoters with dedicated RNA polymerase II or III activity

RNA polymerase III (Pol III) promoters, such as 7SK, U6, and H1, are widely used for the expression of small noncoding RNAs, including short hairpin RNAs for RNAi experiments and guide RNAs for CRISPR-mediated genome editing. We previously reported dual RNA polymerase activity (Pol II/III) for the h...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:J Biol Chem
Main Authors: Gao, Zongliang, van der Velden, Yme Ubeles, Fan, Minghui, van der Linden, Cynthia Alyssa, Vink, Monique, Herrera-Carrillo, Elena, Berkhout, Ben
Format: Artigo
Jezik:Inglês
Izdano: American Society for Biochemistry and Molecular Biology 2020
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7949045/
https://ncbi.nlm.nih.gov/pubmed/33154168
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA120.015386
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