Inhibition of CRISPR-Cas12a DNA targeting by nucleosomes and chromatin

Genome engineering nucleases must access chromatinized DNA. Here, we investigate how AsCas12a cleaves DNA within human nucleosomes and phase-condensed nucleosome arrays. Using quantitative kinetics approaches, we show that dynamic nucleosome unwrapping regulates target accessibility to Cas12a and de...

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Bibliographic Details
Published in:Sci Adv
Main Authors: Strohkendl, Isabel, Saifuddin, Fatema A., Gibson, Bryan A., Rosen, Michael K., Russell, Rick, Finkelstein, Ilya J.
Format: Artigo
Language:Inglês
Published: American Association for the Advancement of Science 2021
Subjects:
Research Articles
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7946368/
https://ncbi.nlm.nih.gov/pubmed/33692102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/sciadv.abd6030
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