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Inhibition of CRISPR-Cas12a DNA targeting by nucleosomes and chromatin
Genome engineering nucleases must access chromatinized DNA. Here, we investigate how AsCas12a cleaves DNA within human nucleosomes and phase-condensed nucleosome arrays. Using quantitative kinetics approaches, we show that dynamic nucleosome unwrapping regulates target accessibility to Cas12a and de...
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| Pubblicato in: | Sci Adv |
|---|---|
| Autori principali: | , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Association for the Advancement of Science
2021
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7946368/ https://ncbi.nlm.nih.gov/pubmed/33692102 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/sciadv.abd6030 |
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