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Kinetic Basis for DNA Target Specificity of CRISPR-Cas12a

Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising applications in human health and disease. However, DNA cleavage at off-target sites that resemble the target sequence is a pervasive problem that remains poorly understood mechanistically. Here, we use quantitative kin...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Mol Cell
Päätekijät: Strohkendl, Isabel, Saifuddin, Fatema A., Rybarski, James R., Finkelstein, Ilya J., Russell, Rick
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2018
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC6679935/
https://ncbi.nlm.nih.gov/pubmed/30078724
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2018.06.043
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