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Rational Selection of CRISPR-Cas9 Guide RNAs for Homology-Directed Genome Editing

Homology-directed repair (HDR) of a DNA break allows copying of genetic material from an exogenous DNA template and is frequently exploited in CRISPR-Cas9 genome editing. However, HDR is in competition with other DNA repair pathways, including non-homologous end joining (NHEJ) and microhomology-medi...

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Dettagli Bibliografici
Pubblicato in:Mol Ther
Autori principali: Tatiossian, Kristina J., Clark, Robert D.E., Huang, Chun, Thornton, Matthew E., Grubbs, Brendan H., Cannon, Paula M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society of Gene & Cell Therapy 2021
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7934447/
https://ncbi.nlm.nih.gov/pubmed/33160457
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymthe.2020.10.006
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